IL10 antibody
Catalogue Number: GTX130513-GTX
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GTX130513 Image
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GTX130513 Image
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An ELISA plate is coated with 50 µL of a IL10 peptide at concentration ranged from 0.25 µg/mL to 8 µg/mL. The coated peptide is detected with anti IL10 antibody (GTX130513) at 0.5 µg/mL.
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IL10 antibody detects IL10 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse hippocampus. Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL10 antibody (GTX130513) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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IL10 antibody detects IL10 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon. IL10 stained by IL10 antibody (GTX130513) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX130513 Image
IL10 antibody detects IL10 protein by immunofluorescent analysis. Sample: Molt-4 cells were fixed in ice-cold MeOH for 5 min. Green: IL10 stained by IL10 antibody (GTX130513) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 21 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | interleukin 10 , CSIF , GVHDS , IL-10 , IL10A , TGIF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human IL10. The exact sequence is proprietary. |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. [provided by RefSeq]
Additional Text
Gene Name
IL10
Gene ID
3586
Uniprot ID
P22301
Concentration
1.17 mg/mL
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
21
Application Notes
WB: 1:1000-1:10000. IHC-Fr: 1:100-1:1000. ELISA: 1:-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX130513 Datasheet
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