NMT1 antibody
Catalogue Number: GTX130852-GTX
GTX130852 Image
GTX130852 Image
GTX130852 Image
GTX130852 Image
GTX130852 Image
GTX130852 Image
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NMT1 antibody (GTX130852) diluted at 1:5000.
GTX130852 Image
NMT1 antibody detects NMT1 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NMT1 antibody (GTX130852) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX130852 Image
NMT1 antibody detects NMT1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NMT1 antibody (GTX130852) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX130852 Image
NMT1 antibody detects NMT1 protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. NMT1 antibody (GTX130852) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX130852 Image
NMT1 antibody detects NMT1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NMT1 protein stained by NMT1 antibody (GTX130852) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 57 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | N-myristoyltransferase 1 , NMT |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human NMT1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM]
Additional Text
Gene Name
NMT1
Uniprot ID
P30419
Concentration
0.42 mg/ml
Purification
Affinity Purified
Gene ID
4836
Molecular Weight
57
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX130852 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations