Troponin T fast skeletal antibody
Catalogue Number: GTX130922-GTX
GTX130922 Image
GTX130922 Image
GTX130922 Image
GTX130922 Image
Human tissue extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNNT3 antibody (GTX130922) diluted at 1:1000.
GTX130922 Image
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNNT3 antibody (GTX130922) diluted at 1:5000.
GTX130922 Image
Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with TNNT3 antibody (GTX130922) diluted at 1:5000.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 32 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | troponin T3, fast skeletal type , TNTF , beta-TnTF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human Troponin T fast skeletal. The exact sequence is proprietary. |
| Application: | WB |
Description
Description: The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq]
Additional Text
Molecular Weight
32
Gene Name
TNNT3
Uniprot ID
P45378
Concentration
0.19 mg/ml
Purification
Affinity Purified
Gene ID
7140
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX130922 Datasheet
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