PHD2 antibody
Catalogue Number: GTX132293-GTX
GTX132293 Image
GTX132293 Image
GTX132293 Image
GTX132293 Image
GTX132293 Image
Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD2 antibody (GTX132293) diluted at 1:2000.
GTX132293 Image
PHD2 antibody detects PHD2 Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
GTX132293 Image
PHD2 antibody detects PHD2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX132293 Image
PHD2 antibody detects PHD2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:500. Blue: Hoechst 33342 staining.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 46 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | egl-9 family hypoxia inducible factor 1 , C1orf12 , ECYT3 , HALAH , HIF-PH2 , HIFPH2 , HPH-2 , HPH2 , PHD2 , SM20 , ZMYND6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PHD2. The exact sequence is proprietary. |
| Application: | ICC, IF, WB, IHC-Fr |
Description
Description: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]
Additional Text
Gene Name
EGLN1
Uniprot ID
Q9GZT9
Concentration
0.99 mg/ml
Purification
Affinity Purified
Gene ID
54583
Molecular Weight
46
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX132293 Datasheet
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