Catalogue Number: GTX133569-GTX
Untreated (-) and treated (+) THP-1 whole cell extract (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody (GTX133569) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (-) and treated (+) Raw264.7 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with NLRP3 antibody (GTX133569) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|Preservative:||0.025% ProClin™ 300|
|Type:||Polyclonal Primary Antibody - Unconjugated|
|Alias:||NLR family pyrin domain containing 3 , AGTAVPRL , AII , AVP , C1orf7 , CIAS1 , CLR1.1 , DFNA34 , FCAS , FCAS1 , FCU , KEFH , MWS , NALP3 , PYPAF1|
|Shipping Condition:||Blue Ice|
|Unit(s):||100 ul, 25 ul|
|Immunogen:||Recombinant protein encompassing a sequence within the N-terminus region of human NLRP3. The exact sequence is proprietary.|
Description: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq]
WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption