CDK2 (phospho Thr160) antibody
Catalogue Number: GTX133862-GTX
GTX133862 Image
GTX133862 Image
GTX133862 Image
GTX133862 Image
GTX133862 Image
Untreated (-) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (GTX133862) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX133862 Image
Wild-type (WT) and CDK2 (phospho Thr160) knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with CDK2 (phospho Thr160) antibody (GTX133862) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX133862 Image
CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (GTX133862) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX133862 Image
CDK2 (phospho Thr160) antibody detects CDK2 (phospho Thr160) protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human colon. CDK2 (phospho Thr160) stained by CDK2 (phospho Thr160) antibody (GTX133862) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 34 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | cyclin dependent kinase 2 , CDKN2 , p33(CDK2) |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding phospho Thr160 of human CDK2. The exact sequence is proprietary. |
| Application: | IHC-P, WB |
Description
Description: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq]
Additional Text
Gene Name
CDK2
Gene ID
1017
Uniprot ID
P24941
Purification
Affinity Purified
Modification
Phosphorylated, Thr160
Concentration
3.35 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
34
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX133862 Datasheet
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