alpha B Crystallin antibody
Catalogue Number: GTX13497-GTX
GTX13497 Image
GTX13497 Image
GTX13497 Image
Immunohistochemical analysis of human brain cortex tissue stained with αB-Crystallin pAb at 10 µg/mL.
GTX13497 Image
Western blot analysis of GTX13497: Lane 1: MW marker, Lane 2: Prod. No. bovine alpha Crystallin protein, Lane 3: bovine alpha A crystallin protein, Lane 4: bovine alpha B crystallin protein, Lane 5: bovine beta crystallin protein.
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 20 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | crystallin alpha B , CMD1II , CRYA2 , CTPP2 , CTRCT16 , HEL-S-101 , HSPB5 , MFM2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide: REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin. |
| Application: | ICC, IF, IP, WB, IHC |
Description
Description: Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Additional Text
Gene ID
1410
Gene Name
CRYAB
Uniprot ID
P02511
Molecular Weight
20
Purification
Protein A purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
Recommended Starting Dilutions:ICC: Use at a dilution of 1/200. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000 - 1/2000. 1/2000 dilution is sufficient to detect 50ng of alpha B Crystallin. Detects a band of approximately 22 kDa (predicted MW 20 kDa). Optimal dilutions/concentrations should be determined by the end user.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX13497 Datasheet
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