SARS-CoV-2 (COVID-19) Spike antibody
Catalogue Number: GTX135356-GTX
GTX135356 Image
GTX135356 Image
GTX135356 Image
GTX135356 Image
GTX135356 Image
GTX135356 Image
GTX135356 Image
GTX135356 Image
GTX135356 Image
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 µg/mL and GTX135356 as detection antibody at concentration of 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX135356 Image
Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) at 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX135356 Image
Indirect ELISA analysis was performed by coating plate with 50 µL of recombinant full-length SARS-CoV-2 (COVID-19) spike protein at concentrations ranging from 0.0625 µg/mL to 4 µg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) at 1 µg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
GTX135356 Image
SARS-CoV-2 (COVID-19) spike antibody detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis. Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block. Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000. Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX135356 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX135356 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX135356 Image
Non-transfected (-) and transfected (+) Vero E6 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX135356 Image
SARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | 2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike |
| Shipping Condition: | Blue Ice |
| Unit(s): | 25 ul, 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary. |
| Application: | ELISA, FC, ICC, IF, IHC-P, sELISA, WB, IHC |
Additional Text
Concentration
0.2 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX135356 Datasheet
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