Nucleolin antibody [4E2]

Catalogue Number: GTX13541-GTX

Manufacturer:GeneTex
Preservative:no preservative|No preservative
Molecular Weight:77
Physical state:Liquid
Type:Monoclonal Primary Antibody - Unconjugated
Alias:nucleolin , C23 , Nsr1
Shipping Condition:Blue Ice
Unit(s): 50 ug
Host name: Mouse
Clone: 4E2
Isotype: IgG1
Immunogen: Human nucleolin protein from Raji cell extract.
Application: ICC, IF, ChIP, WB, IHC

Description

Description: Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Additional Text

Gene Name

NCL

Gene ID

4691

Molecular Weight

77

Purification

Protein A purified

Uniprot ID

P19338

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Concentration

1 mg/ml

Application Notes

ICC: Use at a concentration of 1 - 10 µg/ml. IHC: Use at a concentration of 1 - 10 µg/ml. WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. This antibody allows in situ detection of nucleolar protein in Hep-II cell and paraffin embedded tissue section. Paraffin embedded sections should be processed under microwave treatment. Optimal dilutions/concentrations should be determined by the end user.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.