STAT5A (phospho Tyr694) antibody
Catalogue Number: GTX13593-GTX
GTX13593 Image
GTX13593 Image
GTX13593 Image
GTX13593 Image
GTX13593 Image
IHC-P analysis of mouse spleen tissue using GTX13593 STAT5A (phospho Tyr694) antibody. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20
GTX13593 Image
WB (peptide competition) analysis of 3T3-L1 cells treated with 20 ng/mL LIF for 10 minutes (Lane 2-5) using GTX13593 STAT5A (phospho Tyr694) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
GTX13593 Image
ICC/IF analysis of HeLa cells treated with 5uM of TNF-alpha for 30 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutesDilution: 1:250
GTX13593 Image
ChIP analysis of 2 million HeLa cells treated with 100ng/ml of IFN gamma for 45 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of COX1, AP1 gene as positive control target and the inactive SAT2 used as negative control target.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 91 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | signal transducer and activator of transcription 5A , MGF , STAT5 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human STAT5a that contains tyrosine 694. The sequence is conserved in mouse and rat. |
| Application: | FC, ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]
Additional Text
Gene Name
STAT5A
Gene ID
6776
Uniprot ID
P42229
Purification
Affinity Purified
Modification
Phosphorylated, Tyr694
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
91
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
ICC/IF: 1:250. IHC-P: 1:10-1:100. FCM: 1:20. ChIP assay: 1-5 µg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX13593 Datasheet
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