SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active)

Catalogue Number: GTX136091-PRO-GTX

GTX136091-PRO Image

1 µg of GTX136091-pro SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

GTX136091-PRO Image

SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (0.2 µg, GTX136091-pro) were separated by 12% SDS-PAGE, and the membrane were blotted with various antibodies diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S2 antibody [HL237] (GTX635693) (1 µg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (1 µg/mL). Mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 µg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX136091-PRO Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 µg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) (1 µg/mL)

GTX136091-PRO Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 µg/mL) Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (1 µg/mL)

GTX136091-PRO Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 µg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 µg/mL)

GTX136091-PRO Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below. Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 µg/mL) Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (1 µg/mL)

GTX136091-PRO Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant) using antibodies as below. Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 µg/mL) Detection: HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (1 µg/mL) Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

GTX136091-PRO Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, P.1 / Gamma variant, His tag (active) (GTX136091-pro) (coated at 2 µg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

GTX136091-PRO Image

Functional ELISA analysis of immobilized recombinant (GTX136091-pro) (coated at 2 µg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.15-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

GTX136091-PRO Image

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Manufacturer:	GeneTex
Preservative:	no preservative\|No preservative
Physical state:	Liquid
Type:	Spike Proteins
Alias:	Gamma variant Spike , P.1 Spike , SARS-CoV-2 Gamma variant Spike , COVID-19 Gamma variant Spike
Host Cell:	HEK293 cells
Shipping Condition:	Dry Ice
Unit(s):	100 ug
Application:	ELISA, sELISA, WB, BndA

Overview
Datasheets

Additional Text

Concentration

0.2 mg/ml

Protein Tag

HIS Tag

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Application Notes

Recommended antibody pairs for sandwich ELISA: Capture : GTX635807, Detection : GTX635791 / GTX635793-01 / GTX635792-01 or Capture : GTX632604, Detection : GTX635792 / GTX635672 / GTX635713. Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70C or below. Do not vortex.

GTX136091-PRO Datasheet