Human G-CSF protein (active)
Catalogue Number: GTX138166-PRO-GTX
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GTX138166-PRO Image
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GTX138166-PRO Image
GTX138166-PRO Image
GTX138166-PRO Image
GTX138166-PRO Image
Serial dilutions of Human G-CSF protein (active) were added to murine NFS-60 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.
GTX138166-PRO Image
1 µg of GTX138166-pro Human G-CSF protein (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.
GTX138166-PRO Image
Indirect ELISA analysis was performed by coating the plate with recombinant Human G-CSF protein (active) (GTX138166-pro) (213.9-3.34 nM). Coated protein was probed with G-CSF antibody [GT21] (GTX641067) (1 µg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.
GTX138166-PRO Image
Sandwich ELISA detection of recombinant Human G-CSF protein (active) (GTX138166-pro) using antibodies as below. Capture: G-CSF antibody [GT21] (GTX641067) (5 µg/mL) Detection: HRP-conjugated G-CSF antibody [GT22] (GTX641068) (1 µg/mL) Please notice that GTX641068 needs to be conjugated to HRP to function as the detection antibody when paired with GTX641067. Please contact us for custom HRP-conjugated antibody.
| Manufacturer: | GeneTex |
| Preservative: | No preservative |
| Physical state: | Lyophilized |
| Type: | Growth Factors |
| Alias: | colony stimulating factor 3 , C17orf33 , CSF3OS , GCSF |
| Host Cell: | E.coli |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Application: | ELISA, FA |
Description
Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Additional Text
Gene ID
1440
Gene Name
CSF3
Uniprot ID
P09919
Accession Number
NP_757373.1
Short Description
G-CSF (Granulocyte-colony stimulating factor/CSF3) is the major growth factor driving the generation of mature granulocytes from committed bone marrow myeloid progenitors. This biologically active recombinant human G-CSF is an 18.7 kDa globular protein formed by a 174-amino acid polypeptide with two intramolecular disulfide bonds. Human and murine G-CSFs are highly similar and show reciprocal biological cross-reactivity.
Storage Note
Store at 4C or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70C or below. Do not vortex.
Application Notes
Recommended antibody pairs for sandwich ELISA: Capture: GTX641067 or Detection: GTX641068
GTX138166-PRO Datasheet
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