CENPA antibody [3-19]

Catalogue Number: GTX13939-GTX

Manufacturer:	GeneTex
Preservative:	no preservative\|No preservative
Molecular Weight:	16
Physical state:	Liquid
Type:	Monoclonal Primary Antibody - Unconjugated
Alias:	centromere protein A , CENP-A , CenH3
Shipping Condition:	Blue Ice
Unit(s):	50 ug
Host name:	Mouse
Clone:	3-19
Isotype:	IgG1
Immunogen:	Synthetic peptide: PRRRSRKPEAPRRRSPS, corresponding to amino acids 3-19 of Human CENP A.
Application:	ICC, IF, IHC-P, ChIP, WB, IHC, NT, Inh

Overview
Datasheets

Description

Description: Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. This gene encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2015]

Additional Text

Gene Name

CENPA

Gene ID

1058

Uniprot ID

P49450

Purification

Protein A purified

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Molecular Weight

Concentration

1 mg/ml

Application Notes

ICC: Use at a concentration of 10 µg/ml. IHC: Use at a concentration of 5 µg/ml. For paraffin embedded sections, heat treatment may be necessary. Microwave sections 2 times for 10 minutes each in citrate buffer (pH 6.5). WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). Optimal dilutions/concentrations should be determined by the end user. User review: This antibody is great for immunofluorescence on human cells (primary fibroblasts and transformed cell lines such as HeLa, HT1080, A549, H1299). Works well with various fixation conditions, including 1-4% paraformaldehyde (PFA), -20C methanol, 2% PFA followed by -20C methanol, and on unfixed preparations. We routinely use it at a dilution of 1:200-1:400.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX13939 Datasheet

GTX13939 Reference

Price

£386.00

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