Trident femto Western HRP Substrate
Catalogue Number: GTX14698-GTX
GTX14698 Image
GTX14698 Image
GTX14698 Image
GTX14698 Image
GTX14698 Image
GTX14698 Image
Recombinant protein mcherry is loaded as the follows: Lane A 3.13 ng recombinant protein Lane B 1.56 ng recombinant protein Lane C 780 pg recombinant protein Lane D 390 pg recombinant protein The blot was probed with mcherry antibody (GTX128508) diluted at 1:2000. A HRP-conjugated secondary antibody was applied and developed with 1 ml of Trident femto Western HRP Substrate (GTX14698). Exposure time : 18 seconds, 54 seconds, and 90 seconds. SuperSignalTM is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with GeneTex and does not endorse this product.
GTX14698 Image
Serial dilutions of purified mCherry recombinant protein were prepared and applied at 10 µL/well. After electrophoresis and protein transfer, membranes were probed with anti-mCherry antibody (GTX128508) diluted at 1:1500 and then with goat anti-rabbit HRP-conjugated secondary antibody (1:2000). Identical blots were incubated with 1 ml of Trident femto Western HRP Substrate, SuperSignalTM West Femto, Trident pico Western HRP Substrate, ClarityTM, and LuminataTM Forte. The blots were simultaneously exposed for 90 seconds and 180 seconds using BioSpectrumTM 500 digital imaging system SuperSignalTM West Femto, ClarityTM, LuminataTM Forte are registered trademark of Thermo Fisher Scientific, Bio-Rad, and EMD Millipore, respectively. The trademark holder is not affiliated with GeneTex and does not endorse this product.
GTX14698 Image
GTX14698 Image
Various 293T whole cell extracts were prepared and applied at 10 µL/well. After electrophoresis and protein transfer, membranes were probed with alpha Tubulin antibody (GTX112141) diluted at 1:100000 and then with goat anti-rabbit HRP-conjugated secondary antibody (GTX213110-01). Identical blots were incubated with 1 ml of Trident femto Western HRP Substrate and the mixed substrate (Substrate solution A and Substrate solution B) stored for 0, 15 and 24 hours at 2-8oC. The blots were simultaneously exposed for 18 seconds, 90 seconds and 180 seconds using BioSpectrumTM 500 digital imaging system. The signal was developed with Trident femto Western HRP Substrate (GTX14698).
GTX14698 Image
Recombinant protein mCherry was loaded 100, 20, 4 and 0.8 pg and the blot was probed with mCherry antibody (GTX128508) diluted at 1:500. A HRP-conjugated secondary antibody was applied and developed with 1 ml of Trident femto Western HRP Substrate (GTX14698). Exposure time : 18 and 54 seconds. Immobilon is a registered trademark which is not affiliated with GeneTex and does not endorse this product.
| Manufacturer: | GeneTex |
| Shelf Life: | 1 day |
| Physical state: | Liquid |
| Type: | Western Blotting Reagents |
| Alias: | Trident femto-ECL |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ml, 200 ml |
| Application: | WB |
Description
Description: GeneTex Trident femto Western HRP Substrate can detect horseradish peroxidase at the femtogram level. The substrate is supplied as two components.
Additional Text
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
please refer to GTX14698 Protocol
Storage Note
Trident femto Western HRP Substrate is stable for 18 months at 4C. Keep container tightly closed and store Trident femto Western HRP Substrate away from heat or light. Mixed substrate can be stored for up to 24 hours at 2-8C.
GTX14698 Datasheet
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