Presenilin 2 antibody [APS 21]
Catalogue Number: GTX15548-GTX
GTX15548 Image
GTX15548 Image
GTX15548 Image
GTX15548 Image
GTX15548 Image
GTX15548 Image
IHC-P analysis of human kidney tissue using GTX15548 Presenilin 2 antibody [APS 21]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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WB analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and PC12 (lane 3) using GTX15548 Presenilin 2 antibody [APS 21]. Dilution : 1:200
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ICC/IF analysis of A2058 Cells using GTX15548 Presenilin 2 antibody [APS 21]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
GTX15548 Image
ICC/IF analysis of HeLa cells using GTX15548 Presenilin 2 antibody [APS 21]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
GTX15548 Image
ICC/IF analysis of NCI-H460 Cells using GTX15548 Presenilin 2 antibody [APS 21]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 50 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | presenilin 2 , AD3L , AD4 , CMD1V , PS2 , STM2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ug |
| Host name: | Mouse |
| Clone: | APS 21 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2. |
| Application: | ELISA, ICC, IF, IHC-P, WB, IHC |
Description
Description: Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
PSEN2
Uniprot ID
P49810
Gene ID
5664
Purification
Protein G purified
Molecular Weight
50
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:100-1:1000. ICC/IF: 0.75 µg/ml. IHC-P: 1:100. ELISA: 4.5 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX15548 Datasheet
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