Alpha fetoprotein / AFP antibody [P5B8]
Catalogue Number: GTX15650-GTX
GTX15650 Image
GTX15650 Image
GTX15650 Image
GTX15650 Image
GTX15650 Image
WB analysis of 10 µg of whole cell extract from H9 ESCs, H9 ESC-derived hepatoblasts, ESC-derived hepatocyte-like cells, HepG2 cells, HepaRG cells, and primary human hepatocytes using GTX15650 Alpha fetoprotein / AFP antibody [P5B8]. Dilution : 1 µg/ml
GTX15650 Image
WB analysis of 20 ug of the indicated whole cell lysates using GTX15650 Alpha fetoprotein / AFP antibody [P5B8]. Dilution : 2 µg/ml
GTX15650 Image
ICC/IF analysis of permeabilized endoderm (derived from human ES cells) using GTX15650 Alpha fetoprotein / AFP antibody [P5B8]. Green : Primary antibody Blue : cell nucleus Dilution : 5 µg/ml
GTX15650 Image
ICC/IF analysis of permeabilized endoderm (derived from human ES cells) using GTX15650 Alpha fetoprotein / AFP antibody [P5B8]. Green : Primary antibody Blue : cell nucleus Dilution : 5 µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 69 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | alpha fetoprotein , AFPD , FETA , HPAFP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | P5B8 |
| Isotype: | IgG1 |
| Immunogen: | Purified AFP from Cord Blood |
| Application: | ELISA, ICC, IF, IP, WB, RIA |
Description
Description: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
AFP
Gene ID
174
Uniprot ID
P02771
Molecular Weight
69
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1 µg/ml. ICC/IF: 5 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX15650 Datasheet
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