Apolipoprotein A1 antibody [513]
Catalogue Number: GTX15658-GTX
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IHC-P analysis of human liver tissue sections using GTX15658 Apolipoprotein A1 antibody [513]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : microwaved for 10 minutes in 10mM sodium citrate, pH 6.0 Dilution : 1:50
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WB analysis of various samples using GTX15658 Apolipoprotein A1 antibody [513]. Left Lane : 10ul of serum depleted of highly abundant proteins using the Top 2 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO. Middle Lane : 10ul of serum depleted of highly abundant proteins using the Top 12 Abundant Protein Depletion Spin Columns, and concentrated using the PES Protein Concentrators, 10K MWCO. Right Lane : 50 ug of HepG2 whole cell lysate Dilution : 1:1000
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WB analysis of the indicated amounts of a recombinant human Apo A-1-hIgG1Fc fusion protein or a recombinant mouse Apo A-1-hIgG1Fc fusion protein using GTX15658 Apolipoprotein A1 antibody [513]. Dilution : 1:1000
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Sandwich ELISA analysis of recombinant human Apo A-1 standards ranging from 1.6-1000ng/ml (A) or 100ul of diluted normal human serum or diluted serum from a patient with dyslipidemia (B) using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).
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Sandwich ELISA analysis of recombinant human Apo A-1 or recombinant mouse Apo A-1 protein ranging from 1.6-1000ng/ml using GTX15658 Apolipoprotein A1 antibody [513] (detection antibody) and Apo A-1 rabbit monoclonal antibody (capture antibody).
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 31 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | apolipoprotein A1 , apo(a) |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 513 |
| Isotype: | IgG1 |
| Immunogen: | Purified human plasma Apo A-1 |
| Application: | ELISA, IHC-P, IP, WB, RIA |
Description
Description: This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]
Additional Text
Gene Name
APOA1
Uniprot ID
P02647
Gene ID
335
Purification
Ion Exchange
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
31
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:2000. IHC-P: 1:10-1:100. ELISA: 1µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX15658 Datasheet
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