CREB antibody [LB9]
Catalogue Number: GTX15731-GTX
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IHC-P analysis of human bladder and rectal tissue using GTX15731 CREB antibody [LB9]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Dilution : 1:200
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IHC-P analysis of human tonsil tissue using GTX15731 CREB antibody [LB9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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WB analysis of 25 ug of various whole cell lysates using GTX15731 CREB antibody [LB9]. Dilution : 1:500
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WB analysis of nuclear extracts (30 µg lysate) of Jurkat (Lane 1), HeLa (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), U-2 OS (Lane 7), A549 (Lane 8), MDA-MB-231 (Lane 9) and T98G (Lane10) using GTX15731 CREB antibody [LB9]. Dilution : 1:500
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ICC/IF analysis of MDA-MB-231 cells using GTX15731 CREB antibody [LB9]. Panel e shows the no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 5 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature
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ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:400 for at least 1 hour at roomtemperature
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ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:500 for at least 1 hour at room temperature
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ChIP analysis of 2 million HeLa cells using GTX15731 CREB antibody [LB9]. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to promoter of NR4A3, CYR61 gene used as positive control target, and the MYOD, SAT2 used as negative control target.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 37 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | cAMP responsive element binding protein 1 , CREB , CREB-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | LB9 |
| Isotype: | IgG1 |
| Immunogen: | Recombinant human CREB-A protein. |
| Application: | ELISA, ICC, IF, IHC-P, IP, ChIP, WB, IHC |
Description
Description: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Additional Text
Gene ID
1385
Gene Name
CREB1
Uniprot ID
P16220
Molecular Weight
37
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500. ICC/IF: 5 µg/ml. IHC-P: 1:200. IP: 2 µg. ELISA: 0.1-1 µg/ml. ChIP assay: 2.5 µg/10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX15731 Datasheet
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