ERK1 antibody [12D11]
Catalogue Number: GTX15780-GTX
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GTX15780 Image
GTX15780 Image
GTX15780 Image
GTX15780 Image
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WB analysis of 25 ug of various whole cell lysates using GTX15780 ERK1 antibody [12D11]. Dilution : 1:1000
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ICC/IF analysis of A549 cells using GTX15780 ERK1 antibody [12D11]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature
GTX15780 Image
ICC/IF analysis of untreated and siRNA knockdown treated HeLa cells using GTX15780 ERK1 antibody [12D11]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 for at least 1 hour at room temperature
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IHC-P analysis of human colon tissue using GTX15780 ERK1 antibody [12D11]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95oC
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ChIP analysis of 1x10? HCT116 colon carcinoma cells treated with serum for 0, 15, 30, and 60 minutes using GTX15780 ERK1 antibody [12D11]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to -15kb upstream of the Egr1 gene or exon-1 of Egr1. IP reaction : 1.0ul antibody / 100ul sample
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 43 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | mitogen-activated protein kinase 3 , ERK-1 , ERK1 , ERT2 , HS44KDAP , HUMKER1A , P44ERK1 , P44MAPK , PRKM3 , p44-ERK1 , p44-MAPK |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 12D11 |
| Isotype: | IgG |
| Immunogen: | Full-length human recombinant protein expressed in bacteria. |
| Application: | ICC, IF, IHC-P, ChIP, WB, IHC |
Description
Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
MAPK3
Gene ID
5595
Uniprot ID
P27361
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
43
Concentration
1 mg/ml
Application Notes
WB: 1:1000. ICC/IF: 2 µg/ml. IHC-P: 1:100. ChIP assay: 1-3 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX15780 Datasheet
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