gamma Tubulin antibody [4D11]
Catalogue Number: GTX15794-GTX
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GTX15794 Image
GTX15794 Image
GTX15794 Image
GTX15794 Image
GTX15794 Image
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IHC-P analysis of human breast carcinoma tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
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IHC-P analysis of human colon tissue using GTX15794 gamma Tubulin antibody [4D11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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WB analysis of gamma tubulin knockdown HeLa cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000
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WB analysis of 50 ug of various whole cell lysates using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1:1000
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WB analysis of membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), HepG2 (Lane 4), Caco2 (Lane 5), A-431 (Lane 6), SH-SY5Y (Lane 7) and U-2 OS (Lane 8) using GTX15794 gamma Tubulin antibody [4D11]. Dilution : 1 µg/ml
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ICC/IF analysis of HeLa (A) and U2-OS cells (B) using GTX15794 gamma Tubulin antibody [4D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | tubulin gamma 1 , CDCBM4 , GCP-1 , TUBG , TUBGCP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 4D11 |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein containing residues A A T R P D Y I S W G T Q E Q of human gamma Tubulin. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC |
Description
Description: This gene encodes a member of the tubulin superfamily. The encoded protein localizes to the centrosome where it binds to microtubules as part of a complex referred to as the gamma-tubulin ring complex. The protein mediates microtubule nucleation and is required for microtubule formation and progression of the cell cycle. A pseudogene of this gene is found on chromosome 7. [provided by RefSeq, Jan 2009]
Additional Text
Concentration
1 mg/ml
Gene ID
7283
Gene Name
TUBG1
Uniprot ID
P23258
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
51
Application Notes
WB: 1:1000. ICC/IF: 1:50. IHC-P: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX15794 Datasheet
SUPPORT
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DELIVERY
we offer free delivery to UK universities and non profit organisations