Calreticulin antibody

Catalogue Number: GTX16144-GTX

Manufacturer:	GeneTex
Preservative:	0.08% Sodium azide\|0.08% Sodium azide
Molecular Weight:	48
Physical state:	Liquid
Type:	Polyclonal Primary Antibody - Unconjugated
Alias:	calreticulin , CRT , HEL-S-99n , RO , SSA , cC1qR
Shipping Condition:	Blue Ice
Unit(s):	250 ug
Host name:	Sheep
Clone:
Isotype:	IgG
Immunogen:	C-terminal domain of human calreticulin
Application:	ICC, IF, WB

Overview
Datasheets

Description

Description: Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Additional Text

Gene ID

811

Gene Name

CALR

Uniprot ID

P27797

Molecular Weight

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Concentration

1 mg/ml

Application Notes

For IF: Use at a dilution of 1:50. For WB: Use at a dilution of 1:1,000 - 1:5,000. Predicted molecular weight: 48 kDa. Could be used as a marker for subcellular fractionation of cells. Optimal dilutions/concentrations should be determined by the end user.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

GTX16144 Datasheet

Price

£478.00

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