SRPR alpha antibody
Catalogue Number: GTX16145-GTX
| Manufacturer: | GeneTex |
| Preservative: | 0.08% Sodium azide|0.08% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Srp Receptor Subunit Alpha , Dp-Alpha , Sr-Alpha , Srpr , Srpra |
| Shipping Condition: | Blue Ice |
| Unit(s): | 250 ug |
| Host name: | Sheep |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant fragment, corresponding to amino acids 39-295 of Dog SRPR alpha, which includes part of the amino terminal SRPR beta binding region and the hinge region between it and the carboxyl-terminal GTPase domain. |
| Application: | IP, WB |
Description
Description: The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. SRPR is composed of two subunits SRPR alpha and SRPR beta. The SRPR alpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRPR beta. SRPR alpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRPR alpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRPR alpha has an apparent molecular weight of 72 kDa. SRPR beta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRPR beta is unknown. The membrane spanning domain is at the amino-terminus of SRPR beta. SRPR alpha binds to the GTPase domain of SRPR beta. Heterodimerization of SRPR alpha and SRPR beta masks the carboxyl-terminal epitope of SRPR beta.
Additional Text
Gene ID
489293
Gene Name
SRPRA
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
IP: Use at an assay dependent dilution. Can be used to immunoprecipitate both subunits of SRPR under non-denaturing conditions. WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 72 kDa. Optimal dilutions/concentrations should be determined by the end user.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX16145 Datasheet
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