M13 antibody [E1] (Biotin)
Catalogue Number: GTX17269-GTX
GTX17269 Image
GTX17269 Image
GTX17269 Image
Colo320 cell line stained with a cell-line specific M13 phage antibody (filled profile), compared to an irrelevant phage antibody (open profile). Cells were incubated overnight with 1012 phage particles, then detected with 10ug/ml anti-M13 antibody followed by rabbit anti-mouse IgG:FITC
GTX17269 Image
Data respresents absorbancy readings for A10B phage on rabbit IgG (A10B/IgG), A10B phage on BSA (A10B/BSA), streptavidin on rabbit IgG (SA/IgG) and streptavidin on BSA (SA/BSA) for each dilution of biotinylated anti-M13 monoclonal antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.08% Sodium azide|0.08% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Conjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | E1 |
| Isotype: | IgG2a |
| Immunogen: | Full length M13 phage coat protein; Exact epitope not determined. |
| Application: | ELISA, FC, WB, IHC |
Description
Description: M13 antibody [E1] (GTX17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
Additional Text
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
For ELISA: Use at an assay dependent dilution. For FACS: Use 1 µg/10^6 cells. For WB: Use at a concentration of 1-5 µg/ml. Optimal dilutions/concentrations should be determined by the researcher.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX17269 Datasheet
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