iNOS antibody
Catalogue Number: GTX17504-GTX
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GTX17504 Image
GTX17504 Image
GTX17504 Image
GTX17504 Image
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IHC-P analysis of human heart tissue using GTX17504 iNOS antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GTX17504 Image
IHC-P analysis of human lung tissue using GTX17504 iNOS antibody. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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WB analysis of 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 µg/mL for 16 hours (right lane) using GTX17504 iNOS antibody. Dilution : 1:1000
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ICC/IF analysis of NIH-3T3 cells using GTX17504 iNOS antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of A549 cells using GTX17504 iNOS antibody. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 131 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 200 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr, IHC, IM |
Description
Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
Additional Text
Gene Name
NOS2
UniProt ID
P29477
Gene ID
18126
Molecular Weight
131
Purification
Ammonium Sulfate Precipitated
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:200 - 1:800. ICC/IF: 1:50. IHC-P: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Concentration
4 mg/ml
GTX17504 Datasheet
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DELIVERY
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