Laminin 511 antibody [12D]
Catalogue Number: GTX17688-GTX
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Protein structure of laminin-511
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ELISA anaiysis of seven recombinant human laminins using GTX17688 Laminin 511 antibody [12D].
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IHC-Fr analysis of non-invasive breast carcinoma tissue using GTX17688 Laminin 511 antibody [12D]. Image A : Vascular basement membrane Image B : Mammary gland basement membrane Image C : Basement membrane-like structures surrounding tumor cells
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IHC-Fr analysis of lung cancer (SCC and ADC) tissue using GTX17688 Laminin 511 antibody [12D]. Left panel : Lung squamous cell carcinoma (SCC) Right panel : lung adenocarcinoma (ADC)
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IHC-Fr analysis of normal mammary gland using GTX17688 Laminin 511 antibody [12D]. Black arrows : Vascular basement membrane White arrowheads : Mammary gland basement membrane
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WB analysis of recombinant human intact laminin-511 (Lm511, containing consisting of alpha5, beta 1 and gamma 1 chains ) protein under non-reducing conditions using GTX17688 Laminin 511 antibody [12D]. Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | no preservative|No preservative |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 12D |
| Isotype: | IgG2b |
| Immunogen: | Human placental laminin. |
| Application: | AP, ELISA, IP, WB, IHC-Fr |
Additional Text
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Application Notes
WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
Laminins, which consist of three subunits called alpha, beta and gamma chains, are major cell-adhesive components of extracellular matrix, especially basement membranes (BMs). The laminin family is constituted of over 15 isoforms, and each member is expressed in a tissue-specific manner and plays a differential role in each tissue. Laminin-511 (Lm511) (formerly laminin-10), which consists of alpha 5, beta 1 and gamma 1 chains, appears at an early stage of development and is most widely expressed in adult tissues, mainly basement membranes of various epithelial tissues and vasculatures. Recently, laminin-511 is often used as a culture substrate for ES and iPS cells.This antibody specifically interacts with the trimeric structure of laminin-511 isoform but does not react with any of the alpha 5, beta 1 and gamma 1 chains in the reducing conditions. Furthermore, it has been validated that clone 12D does not react with any of laminins-111, -211, -332, -3B32, -411 and -521. Unlike conventional anti- alpha 5 antibodies, clone 12D distinguishes laminin-511 from laminin-521. This is an important tool to evaluate specific distribution and physiological functions of laminin-511. In human cancers, clone 12D detects small vascular vessels with high sensitivity and hence useful for evaluating tumor angiogenesis.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX17688 Datasheet
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