IKK alpha (phospho Ser176/Ser180) antibody
Catalogue Number: GTX17943-GTX
GTX17943 Image
GTX17943 Image
GTX17943 Image
GTX17943 Image
WB analysis of Jurkat cells stimulated with TNF-alpha (Lane 1-4, 6) or untreated (Lane 5) using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. The primary antibody was prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphoserine containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) Dilution : 1:500
GTX17943 Image
ICC/IF analysis of HeLa cells treated for 20 minutes with 50ng/ml of TNF-Alpha using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. Fixation : 4% paraformaldehyde Permeabilization : 0.25% Triton X-100 for 10 minutes Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature
GTX17943 Image
ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX17943 IKK alpha (phospho Ser176/Ser180) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of IL-8 gene and the inactive SAT2 used as negative control target.
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 85 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | component of inhibitor of nuclear factor kappa B kinase complex , IKBKA , IKK-alpha , IKK1 , IKKA , NFKBIKA , TCF16 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human IKK-alpha that contains serine 176 and serine 180. |
| Application: | FC, ICC, IF, ChIP, WB |
Description
Description: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
CHUK
Gene ID
1147
Uniprot ID
O15111
Molecular Weight
85
Modification
Phosphorylated, Ser176
Purification
Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:250. ICC/IF: 1:250. FCM: 1:20. ChIP assay: 10µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX17943 Datasheet
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