Androgen Receptor antibody, N-term
Catalogue Number: GTX19066-GTX
GTX19066 Image
GTX19066 Image
GTX19066 Image
GTX19066 Image
GTX19066 Image
GTX19066 Image
ChIP analysis of DHT-treated HEC50 cells using GTX19066 Androgen Receptor antibody, N-term. The precipitated DNA was detected by PCR with primer set targeting to FKBP5. ChIP reaction : 2µg antibody / 1 µg chromatin
GTX19066 Image
WB analysis of human cerebellum (A) and negative control pancreas (B) lysate using GTX19066 Androgen Receptor antibody, N-term. Dilution : 0.5µg/ml Loading : 35µg protein in RIPA buffer
GTX19066 Image
IHC-P analysis of human prostate using GTX19066 Androgen Receptor antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 2µg/ml
GTX19066 Image
ICC/IF analysis of PFA fixed MCF7 cells using GTX19066 Androgen Receptor antibody, N-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX19066 Image
ICC/IF analysis of PFA fixed U2OS cells using GTX19066 Androgen Receptor antibody, N-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 99 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2. |
| Application: | ICC, IF, IHC-P, ChIP, WB |
Description
Description: The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Additional Text
Gene Name
AR
Gene ID
367
Uniprot ID
P10275
Concentration
0.5 mg/ml
Molecular Weight
99
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 0.1-0.5µg/ml. ICC/IF: 10µg/ml. IHC-P: 2-3µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX19066 Datasheet
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