Collagen I antibody
Catalogue Number: GTX20292-GTX
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IHC-P analysis of human lung tissue section using GTX20292 Collagen I antibody. Antigen retrieval : user optimized Dilution : 1:400 Localization : Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Staining : antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
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IHC-P analysis of normal kidney tissue section using GTX20292 Collagen I antibody. Antigen retrieval : No antigen retrieval was performed. Dilution : 1:100 Localization : Distal tubules in normal kidney tissue. Note : the absence of staining of glomeruli.
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Western blot of Human Collagen Type I. Load: 50 ng Human Collagen Type 1. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4oC. Secondary antibody: DyLight? 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa.
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The data was published in the journal Front Pharmacol in 2016. PMID: 27199755
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The data was published in the journal J Cell Mol Med in 2019.PMID: 30411499
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IHC-P analysis of human lung tissue using GTX20292 Collagen I antibody. Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative. Dilution : 1:400
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IHC-P analysis of liver tissue using GTX20292 Collagen I antibody. Panel A : Central Vein (CV) fibrosis Panel B : Non-fibrotic Central Vein (CV) Panel C : Perisinusodial fibrosis Panel D : Non-fibrotic area Panel E : Protat tract fibrosis Panel F : Septal fibrosis (arrow) Antigen retrieval: not performed. Dilution : 1:1250
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IHC-P analysis of normal mouse kidney tissue using GTX20292 Collagen I antibody. Antigen retrieval: not performed. Dilution : 1:100
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WB analysis of human Collagen Type I protein using GTX20292 Collagen I antibody. Loading : 50 ng Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 139 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Collagen Type I from human and bovine placenta |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, WB, DB, Multiplex |
Description
Description: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
Additional Text
Gene Name
COL1A1
Uniprot ID
P02452
Purification
Affinity Purified
Gene ID
1277
Molecular Weight
139
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. IHC-P: 1:50-1:200. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX20292 Datasheet
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