Collagen II antibody
Catalogue Number: GTX20300-GTX
GTX20300 Image
GTX20300 Image
GTX20300 Image
GeneTex anti collagen II antibody (GTX20300, 1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100oC - 20 minutes for antigen retrieval.
GTX20300 Image
The data was published in the journal Oxid Med Cell Longev in 2015.PMID: 26273418
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 142 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | collagen type II alpha 1 chain , ANFH , AOM , COL11A3 , SEDC , STL1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Collagen Type II from human knee cartilage and bovine nasal cartilage. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, DB, IHC, Multiplex |
Description
Description: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.
Additional Text
Gene Name
COL2A1
Uniprot ID
P02458
Gene ID
1280
Purification
Affinity Purified
Molecular Weight
142
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. IHC-P: 1:50-1:400. IP: 1:100. ELISA: 1:5000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX20300 Datasheet
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