Antigen Retrieval Solution

Catalogue Number: GTX20970-GTX

Manufacturer:	GeneTex
Physical state:	Liquid
Type:	Immunodetection Kits and Reagents
Alias:	Antigen retrieval solution - enzymatic ( trypsin )
Shipping Condition:	Blue Ice
Unit(s):	1 kit
Application:	IHC-P

Overview
Datasheets

Description

Description: Trypsin is a commonly used digestive enzyme. In formalin-fixed paraffin-embedded tissues, certain antibody protocols required enzyme pretreatment for proper immunohistochemical staining. This product used to be called ""Carezyme Trypsin Enzymatic Antigen Retrieval Solution"".

Additional Text

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

Deparaffinize tissues and hydrate to water. If necessary perform hydrogen peroxide block, was in water and rinse in PBS or TBS. The tissue section is now ready for trypsin digestion. Combine 1 part trypsin with 1 part buffer. Incubate for 5-10 miinutes at room temperature. Rinse well in PBS or TBS buffer and proceed with immunostaining procedure.This product utilises - color-coded buffer (rose) for easy identification. The buffer incorporates a color-coded pH indicator. The end-user can visually inspect the solution and see that the mixture or buffer is at the proper pH. If the mixed solution is purple, the pH is too high for optimum digestion. If the buffer or trypsin solution turns orange or yellow, the pH is too low for optimum digestion. This product is provided as a two component, ready-to-use solution, and can be used at different concentrations: (1) 25 ml Concentrated liquid trypsin (2) 25 ml of trypsin buffer

Short Description

Antigen retrieval solution-enzymatic (trypsin)

GTX20970 Datasheet

Price

£242.00

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