GFP antibody [9F9.F9]
Catalogue Number: GTX21218-GTX
GTX21218 Image
GTX21218 Image
GTX21218 Image
GTX21218 Image
GTX21218 Image
GTX21218 Image
GTX21218 Image
Figure: Western blot of GFP recombinant protein detected with GeneTex monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. GTX21218 detects a 27 kDa band corresponding to the epitope tag GFP. The membrane was probed with the primary antibody diluted to 1.0 µg/ml for 1 h at room temperature followed by washes and reaction with a 1:2,500 dilution of IRDyeR 800 conjugated goat anti-Mouse IgG [H&L]. IRDyeR 800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
GTX21218 Image
GTX21218 Image
IP analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : GFP input Antibody amount : 10 µg
GTX21218 Image
WB analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Dilution : 1:400
GTX21218 Image
WB analysis of various samples using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : 50 ng GFP Lane 3 : 20 ng GFP Lane 4 : 10 ng GFP Lane 5 : Mock Dilution : 1:1000
GTX21218 Image
WB analysis of recombinant GFP protein using GTX21218 GFP antibody [9F9.F9]. Lane 1 : Protein ladder Lane 2 : 50 ng GFP protein Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 9F9.F9 |
| Isotype: | IgG |
| Immunogen: | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. |
| Application: | ELISA, FC, ICC, IF, IP, ChIP, WB, IHC-Fr, DB, IHC, Multiplex |
Description
Description: The jellyfish Aequorea victoria contains green fluorescent protein (GFP) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expression and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
Additional Text
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:3000-1:30000. ICC/IF: Assay dependent. IHC-Fr: 1:1000-1:5000. FCM: Assay dependent. IP: Assay dependent. ELISA: 1:10000-1:30000. IHC: 1:1000-5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX21218 Datasheet
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