SMC1 (phospho Ser966) antibody
Catalogue Number: GTX21276-GTX
GTX21276 Image
GTX21276 Image
GTX21276 Image
GTX21276 Image
GTX21276 Image
GTX21276 Image
WB analysis of HeLa cells (30 µg/lane) treated with 10 Gy ionizing irradiation using GTX21276 SMC1 (phospho Ser966) antibody.
GTX21276 Image
WB analysis of NCS treated(+; 200 ng/ml; 1h) or mock treated (-) HeLa cell using GTX21276 SMC1 (phospho Ser966) antibody. Dilution : 0.1 µg/ml
GTX21276 Image
ICC/IF analysis of Neocarzinostatin treated asynchronous HeLa cells using GTX21276 SMC1 (phospho Ser966) antibody. Red : Primary antibody Blue : DAPI
GTX21276 Image
IHC-P analysis of human breast carcinoma tissue using GTX21276 SMC1 (phospho Ser966) antibody. Dilution : 1 µg/ml
GTX21276 Image
PLA analysis of HeLa cells using GTX21276 SMC1 (phospho Ser966) antibody and anti-human SA1 antibody. Red : positive signal Blue : DAPI
| Manufacturer: | GeneTex |
| Preservative: | 0.09% sodium azide |
| Molecular Weight: | 143 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, PLA |
Description
Description: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq]
Additional Text
Gene ID
8243
Gene Name
SMC1A
Uniprot ID
Q14683
Purification
Affinity Purified
Modification
Phosphorylated, Ser966
Molecular Weight
143
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500 - 1:5000. ICC/IF: 1:250 - 1:1000. IHC-P: 1:500 - 1:2000. IP: 2 - 6 µg/mg lysate. ELISA: 1:100 - 1:2000. PLA: 1:1000 - 1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4C. DO NOT FREEZE.
GTX21276 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations