Horseradish Peroxidase antibody

Catalogue Number: GTX22116-GTX

Manufacturer:GeneTex
Preservative:no preservative|No preservative
Physical state:Liquid
Type:Polyclonal Primary Antibody - Unconjugated
Shipping Condition:Blue Ice
Unit(s): 100 ul
Host name: Sheep
Clone:
Isotype: IgG
Immunogen: Horseradish peroxidase
Application: ELISA, WB

Description

Description: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric.

Additional Text

Concentration

10 mg/mL

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

WB: 1:500-1:3000. ELISA: 1:2000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.