EasyBlot anti Mouse IgG (HRP)

Catalogue Number: GTX221667-01-GTX

Manufacturer:GeneTex
Preservative:0.025% ProClin 300|0.025% ProClin 300
Physical state:Liquid
Type:Polyclonal Secondary Antibody - Conjugated
Shipping Condition:Blue Ice
Unit(s): 250 ul, 50 ul
Host name: Goat
Clone:
Isotype: IgG
Immunogen:
Application: WB

Additional Text

Purification

Affinity Purified

Concentration

0.03 mg/ml

Antibody Clonality

Polyclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Short Description

EasyBlot anti-Mouse IgG is an HRP-conjugated second step reagent that specifically reacts with the native, non-reduced form of mouse IgG and does not bind the reduced, denatured form. Using Easyblot decreases the interference caused by the heavy (~50 kDa) and light chains (~25 kDa) of the IgG used for immunoprecipitation (IP). EasyBlot is simple to use and results in Western Blot detection of only the target band in your IP experiments. Exercise caution when using Protein A/G for IP with EasyBlot. Protein A/G contaminants bind to mouse IgG with high affinity and may cause contaminating bands with a molecular weight similar to IgG heavy chain or light chian. To minimize background from Protein A/G contamination, we strongly recommend the use of EasyBlot Kit anti-Mouse IgG (Optimized for Prot A/G) (GTXGTX225857-01) or EasyBloker (GTX425858) to minimize protein A/G contamination or you can use anti-Mouse IgG Agarose beads (GTX27018) instead of Protein A/G.

Storage Note

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.