Merlin (phospho Ser518) antibody
Catalogue Number: GTX22478-GTX
GTX22478 Image
GTX22478 Image
GTX22478 Image
Affinity purified phospho-specific antibody to NF2 (Merlin) at pS518 (GTX22478) was used at a 1:1000 dilution to detect NF2 by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lane 1) or CLA treated at 4nM, 20 nM or 100 nM (lanes 2, 3 and 4 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system. Other detection methods will yield similar results.
GTX22478 Image
WB analysis of mouse cardiac myocyte lysate using GTX22478 Merlin (phospho Ser518) antibody. Lane 1 : Mock Lane 2 : 4 nM CLA-treated cells Lane 3 : 20 nM CLA-treated cells Lane 4 : 100 nM CLA-treated cells Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 70 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | neurofibromin 2 , ACN , BANF , SCH |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to KLH. |
| Application: | ELISA, ICC, IF, IHC-P, WB, Multiplex |
Description
Description: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERMs suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and detected as a single band of about 75kD. This antibody is specific for the phosphorylated form of human NF2 (Merlin).
Additional Text
Gene ID
4771
Gene Name
NF2
Uniprot ID
P35240
Purification
Affinity Purified
Modification
Phosphorylated, Ser518
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
70
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:2000. ELISA: 1:200000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22478 Datasheet
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