Myosin Light Chain 2 (phospho Ser19) antibody
Catalogue Number: GTX22480-GTX
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Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at 2.5 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows strong staining of both vascular and myometrial smooth muscle cells of the uterus. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
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Myosin Light chain (phospho Ser20) antibody (GTX22480) was used at a 1:5000 dilution to detect myosin light chain by Western blot. Either 13 or 20 ul of a mouse cardiac myocyte lysate was loaded on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock-treated or CLA-treated, as indicated. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system
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Western blot of Myosin Light chain (phospho Ser20) antibody (GTX22480). Lane 1: Regulatory Light Chain Non-Phospho recombinant protein. Lane 2: Regulatory Light Chain Phospho recombinant protein. Lane 3: Smooth Muscle Non-Phospho recombinant protein. Lane 4: Smooth Muscle Phospho recombinant protein. Load: 50 ng per lane. Primary antibody at 1:1,000 overnight at 4oC. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 60 min at RT. Blocking for 30 min at RT. Predicted/Observed size: 20 kDa.
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The data was published in the journal J Neurosci in 2017.PMID: 28729440
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The data was published in the journal Development in 2015.PMID: 25715397
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ELISA analysis of BSA-conjugated non- and phospho-peptides using serially diluted GTX22480 Myosin Light Chain 2 (phospho Ser19) antibody. Red : Phosphorylated peptide Green : Non-phosphorylated peptide Coating : 0.1 µg
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IHC-P analysis of mouse heart muscle tissue using GTX22480 Myosin Light Chain 2 (phospho Ser19) antibody. Dilution : 1:100
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WB analysis of various recombinant proteins using GTX22480 Myosin Light Chain 2 (phospho Ser19) antibody. Lane 1 : Regulatory Light Chain Non-Phospho recombinant protein Lane 2 : Regulatory Light Chain Phospho recombinant protein Lane 3 : Smooth Muscle Non-Phospho recombinant protein Lane 4 : Smooth Muscle Phospho recombinant protein Laoding : 50 ng Dilution : 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | myosin light chain 12A , HEL-S-24 , MLC-2B , MLCB , MRCL3 , MRLC3 , MYL2B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Human Myosin Light Chain phospho peptide corresponding to a region near the amino terminus of the human smooth/non-muscle form of myosin regulatory light chain conjugated to KLH. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, IHC-Fr, Multiplex |
Description
Description: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium levels and myosin phosphorylation, resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. This antibody is specific for the phosphorylated form of myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human and mouse have almost identical sequences. In human the phosphorylation site is pS19, while in mouse the site maps to pS20.
Additional Text
Purification
Affinity Purified
Modification
Phosphorylated, Ser19
Concentration
1.19 mg/ml
Gene ID
103910; 10627
Uniprot ID
O14950, P19105
Gene Name
MYL12A, MYL12B
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:2000. IHC-P: 2-5 µg/mL. IP: 1:100. ELISA: 1:10000-1:30000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22480 Datasheet
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