NFAT1 antibody [25A10.D6.D2]
Catalogue Number: GTX22722-GTX
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IHC-P analysis of human colon carcinoma tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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IHC-P analysis of human spleen tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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IHC-P analysis of human tonsil tissue using GTX22722 NFAT1 antibody [25A10.D6.D2]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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ICC/IF analysis of HeLa cells untreated (left panel) or treated with 1uM staurosporine (right panel) for 3 hours using GTX22722 NFAT1 antibody [25A10.D6.D2]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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ChIP analysis of 1x10? HTC-IR rat hepatoma cells treated with insulin for 0, 10, and 30 minutes using GTX22722 NFAT1 antibody [25A10.D6.D2]. Immunoprecipitation was performed using a multiplex microplate Matrix ChIP assay (refer to PMID: 22098709). The precipitated DNA was detected by PCR with primer set targeting to exon-1 or exon-28 of the LAMC1 gene. IP reaction : 1.0ul antibody / 100ul sample
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ICC/IF analysis of MCF-7 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of HeLa cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of U251 cells using GTX22722 NFAT1 antibody [25A10.D6.D2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 100 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 , AI607462 , NF-ATc2 , NF-ATp , NFAT1 , NFAT1-D , Nfatp |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 25A10.D6.D2 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to residues A(51) I S S P S G L A Y P D D V L D Y G L(69) of mouse NFATc2-A, B and C isoforms. |
| Application: | FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr, EMSA |
Description
Description: The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that collectively regulate the immune response. Four NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or NFATc1), NFAT3, and NFAT4 (NFATx or NFATc3). These proteins show a low level of sequence similarity with the Dorsal/Rel/NFkB family of transcription factors. Another NFAT-related protein termed NFAT5 differs from isoforms 1-4 in that it lacks many of the Fos/Jun contact sites observed in its predecessors and its subcellular localization is not calcineurin-dependent.
Additional Text
Gene Name
NFATC2
Uniprot ID
Q60591
Purification
Protein G purified
Molecular Weight
100
Gene ID
18019
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1 µg/ml. ICC/IF: 1:100 - 1:1000. IHC-P: 1:50-1:200. IHC-Fr: 1:50-1:200. ChIP assay: 1-3 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22722 Datasheet
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