Clathrin heavy chain antibody [X22]
Catalogue Number: GTX22731-GTX
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IHC-P analysis of human breast carcinoma tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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IHC-P analysis of human colon tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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WB analysis of 25 ug of human brain lysates using GTX22731 Clathrin heavy chain antibody [X22]. Dilution : 1:300
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The data was published in the journal Sci Rep in 2019.PMID: 30787373
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ICC/IF analysis of HeLa cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
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ICC/IF analysis of NCI-H460 Cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
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ICC/IF analysis of U251 cells using GTX22731 Clathrin heavy chain antibody [X22]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 192 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | clathrin heavy chain , CHC , CHC17 , CLH-17 , CLTCL2 , Hc , MRD56 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | X22 |
| Isotype: | IgG1 |
| Immunogen: | Purified human brain clathrin heavy chain. |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC, NT, Inh, IM |
Description
Description: Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
CLTC
Uniprot ID
Q00610
Concentration
6 mg/ml
Gene ID
1213
Molecular Weight
192
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:100-1:500. ICC/IF: 1:1,000. IHC-P: 1:100. FCM: 1/200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22731 Datasheet
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