Glucocorticoid Receptor antibody [BuGR2]
Catalogue Number: GTX22768-GTX
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FACS analysis of Hela cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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FACS analysis of Jurkat cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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FACS analysis of NIH/3T3 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2] compared to an isotype control (blue). Dilution : 2 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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WB analysis of mouse liver extract using GTX22768 Glucocorticoid Receptor antibody [BuGR2].
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ICC/IF analysis of A549 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
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ICC/IF analysis of HeLa cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
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ICC/IF analysis of U251 cells using GTX22768 Glucocorticoid Receptor antibody [BuGR2]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 88 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | nuclear receptor subfamily 3, group C, member 1 , GR , Gcr , Grl |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | BuGR2 |
| Isotype: | IgG2a |
| Immunogen: | Partially purified rat GR. |
| Application: | ELISA, FC, ICC, IF, IHC-P, IP, ChIP, WB, EMSA |
Description
Description: glucocorticoid receptor that binds and activates hormone-dependent transcriptional enhancers [RGD, Feb 2006]
Additional Text
Gene Name
NR3C1
Molecular Weight
88
Gene ID
24413
Purification
Protein A purified
Uniprot ID
P06536
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 5 µg/ml. ICC/IF: 1:50-1:500. IHC-P: 5 µg/ml. FCM: 1-2 µg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22768 Datasheet
SUPPORT
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PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations