AHR antibody [RPT9]
Catalogue Number: GTX22769-GTX
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FACS analysis of HeLa cells using GTX22769 AHR antibody Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature[RPT9] compared to an isotype control (blue).
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FACS analysis of NIH/3T3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue). Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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FACS analysis of PC-3 cells using GTX22769 AHR antibody [RPT9] compared to an isotype control (blue). Dilution : 1:50 for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature
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WB analysis of 20 µg of HEK293 lysate overexpressing Aryl Hydrocarbon Receptor (Lane B) or empty vector control (Lane A) using GTX22769 AHR antibody [RPT9]. Dilution : 1:1000
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ICC/IF analysis of HeLa Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of U251 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of A2058 Cells using GTX22769 AHR antibody [RPT9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Aryl hydrocarbon receptor |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | RPT9 |
| Isotype: | IgG3 |
| Immunogen: | Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of mouse AhR. |
| Application: | ELISA, FC, ICC, IF, IP, ChIP, WB, IHC-Fr, IHC, EMSA |
Description
Description: binds 3-methylcholanthrene and other aromatic hydrocarbons; may play a role in bone formation [RGD, Feb 2006]
Additional Text
Gene Name
AHR
Gene ID
25690
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:500 - 1:2000. ICC/IF: 1:10-1:100. FCM: 1:50. ChIP assay: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22769 Datasheet
SUPPORT
outstanding technical support
PRODUCT
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DELIVERY
we offer free delivery to UK universities and non profit organisations