AHR antibody [RPT1]
Catalogue Number: GTX22770-GTX
GTX22770 Image
GTX22770 Image
GTX22770 Image
GTX22770 Image
IHC-P analysis of human breast carcinoma tissue using GTX22770 AHR antibody [RPT1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
GTX22770 Image
WB analysis of 20 ug of HepG2, U2OS, and K562 whole cell lysates using GTX22770 AHR antibody [RPT1]. Dilution : 1:1000
GTX22770 Image
ICC/IF analysis of NIH-3T3 cells using GTX22770 AHR antibody [RPT1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 96 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | aryl hydrocarbon receptor , bHLHe76 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | RPT1 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to residues R(12) K R R K P(17) V(22) K P I P A E G I K(31) of human AhR. |
| Application: | ELISA, ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]
Additional Text
Gene Name
AHR
Uniprot ID
P35869
Gene ID
196
Molecular Weight
96
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500 - 1:2000. ICC/IF: 1:100-1:1000. IHC-P: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22770 Datasheet
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