HIF1 beta antibody [2B10]
Catalogue Number: GTX22771-GTX
GTX22771 Image
GTX22771 Image
GTX22771 Image
GTX22771 Image
GTX22771 Image
GTX22771 Image
WB analysis of whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), NIH/3T3 (lane 3) and PC 12 (Lane 4) using GTX22771 HIF1 beta antibody [2B10]. Dilution : 1:500-3000
GTX22771 Image
ICC/IF analysis of NRK cells using GTX22771 HIF1 beta antibody [2B10]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 40 µg/ml in staining buffer for 1 hour at room temperature
GTX22771 Image
ICC/IF analysis of A2058 Cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
GTX22771 Image
ICC/IF analysis of HeLa cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
GTX22771 Image
ICC/IF analysis of U251 cells using GTX22771 HIF1 beta antibody [2B10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 87 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | aryl hydrocarbon receptor nuclear translocator , HIF-1-beta , HIF-1beta , HIF1-beta , HIF1B , HIF1BETA , TANGO , bHLHe2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 2B10 |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to residues C N(771) S Y N N E E F P D L T M F P P F S E(789) of human ARNT. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC, EMSA |
Description
Description: This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
Additional Text
Gene ID
405
Gene Name
ARNT
Uniprot ID
P27540
Molecular Weight
87
Purification
Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:1,000. IHC-P: 5 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22771 Datasheet
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