PPAR alpha antibody [3B6/PPAR]
Catalogue Number: GTX22779-GTX
GTX22779 Image
GTX22779 Image
GTX22779 Image
GTX22779 Image
GTX22779 Image
GTX22779 Image
FACS analysis of 3T3 cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of Hela cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of Jurkat cells using GTX12412 PPAR alpha antibody [3B6/PPAR] compared to an isotype control (blue). Dilution : 0.25 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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WB analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) using GTX22779 PPAR alpha antibody [3B6/PPAR]. Dilution : 1:1000 (Hela and Jurkat) and 1:500 (NIH-3T3)
GTX22779 Image
ICC/IF analysis of HCT116 cells using GTX22779 PPAR alpha antibody [3B6/PPAR]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 52 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | peroxisome proliferator activated receptor alpha , NR1C1 , PPAR , PPARalpha , hPPAR |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 3B6/PPAR |
| Isotype: | IgG2b |
| Immunogen: | Purified recombinant PPAR alpha protein. |
| Application: | FC, ICC, IF, IP, ChIP, WB, EMSA |
Description
Description: Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
PPARA
Gene ID
5465
Uniprot ID
Q07869
Molecular Weight
52
Purification
Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 2-5 µg/ml. ICC/IF: 2µg/ml. FCM: 1µg per 10⁶ cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22779 Datasheet
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