Endothelin 1 antibody [TR.ET.48.5]
Catalogue Number: GTX22786-GTX
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FACS analysis of 293T cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of 3T3 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of HepG2 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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WB analysis of 25 ug of PC12 cell lysate using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Dilution : 1:500
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ICC/IF analysis of Hela cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
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ICC/IF analysis of HUVEC cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
GTX22786 Image
ICC/IF analysis of PC12 cells using GTX22786 Endothelin 1 antibody [TR.ET.48.5]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 24 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | endothelin 1 , ARCND3 , ET1 , HDLCQ7 , PPET1 , QME |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | TR.ET.48.5 |
| Isotype: | IgG1 |
| Immunogen: | Pure endothelin-1 conjugated to KLH. |
| Application: | FC, ICC, IF, IHC-P, WB, RIA, IHC-Fr |
Description
Description: This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Additional Text
Uniprot ID
P05305
Gene Name
EDN1
Molecular Weight
24
Gene ID
1906
Concentration
2.5 mg/mL
Purification
Affinity Purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:100-1:1000. ICC/IF: 1:200-1:1000. IHC-P: 1:250. IHC-Fr: 1:250. FCM: 0.5 µg/test. RIA: 1:25,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22786 Datasheet
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