Hsp70 antibody [5A5]
Catalogue Number: GTX22787-GTX
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IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5]. GTX22787 was used as IP and IB antibody IP reaction : 2µg antibody / 500µg lysate WB antibody Dilution : 1:1000
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WB analysis of 50µg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5]. Dilution : 1:1000
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ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
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ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
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ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
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ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 250 ul |
| Host name: | Mouse |
| Clone: | 5A5 |
| Isotype: | IgG1 |
| Immunogen: | Human recombinant HSP70 over expressed in E. coli. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC, NT, EMSA, Inh |
Additional Text
Gene ID
3303; 3304
Purification
Protein A purified
Uniprot ID
P0DMV8, P0DMV9
Gene Name
HSPA1A, HSPA1B
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Specificity
heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70
Concentration
1 mg/ml
Application Notes
WB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22787 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations