HSP27 antibody [G3.1]
Catalogue Number: GTX22790-GTX
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IHC-P analysis of human breast carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500
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IHC-P analysis of human colon carcinoma tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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IHC-P analysis of human tonsil tissue using GTX22790 HSP27 antibody [G3.1]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:500
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IP analysis of HeLa cell lysates using GTX22790 HSP27 antibody [G3.1]. GTX22790 was used as IP and IB antibody IP reaction : 2µg antibody / 500µg lysate WB antibody dilution : 1:1000
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WB analysis of 50µg of the indicated whole cell lysates using GTX22790 HSP27 antibody [G3.1]. Dilution : 1:1000
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ICC/IF analysis of HeLa cells and negative control NIH3T3 cells using GTX22790 HSP27 antibody [G3.1]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
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ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature
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ICC/IF analysis of C6 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC
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ICC/IF analysis of HeLa cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
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ICC/IF analysis of U251 cells using GTX22790 HSP27 antibody [G3.1]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 23 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | heat shock protein family B (small) member 1 , CMT2F , HEL-S-102 , HMN2B , HS.76067 , HSP27 , HSP28 , Hsp25 , SRP27 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | G3.1 |
| Isotype: | IgG1 |
| Immunogen: | Partially purified human HSP27. |
| Application: | ELISA, ICC, IF, IHC-P, IP, WB, IHC, IM |
Description
Description: This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]
Additional Text
Gene Name
HSPB1
Gene ID
3315
Uniprot ID
P04792
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
23
Application Notes
WB: 1:500 - 1:1000. ICC/IF: 1:50 - 1:500. IHC-P: 1:500. IP: 2 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22790 Datasheet
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