GRP94 antibody [9G10]
Catalogue Number: GTX22791-GTX
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GTX22791 Image
GTX22791 Image
GTX22791 Image
GTX22791 Image
GTX22791 Image
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IHC-P analysis of mouse breast tissue using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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IHC-P analysis of mouse liver tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GTX22791 Image
IHC-P analysis of mouse lymph node tissue using GTX22791 GRP94 antibody [9G10]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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WB analysis of 2-fold serial dilutions of HeLa cell lysate(starting at 10 ug, per well) using GTX22791 GRP94 antibody [9G10]. Dilution : 1:1000
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WB analysis of 25 ug of Hela (lane 1), C6 (lane 2) and NIH-3T3 (lane 3) lysates using GTX22791 GRP94 antibody [9G10]. Dilution : 1:200
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ICC/IF analysis of U2OS cells using GTX22791 GRP94 antibody [9G10]. Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes Dilution : 1:100 for at least 1 hour at room temperature
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IHC-P analysis of poorly differentiated adenocarcinoma tissue from human colon tissue sections using GTX22791 GRP94 antibody [9G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95oC Dilution : 1:100
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ICC/IF analysis of C6 cells using GTX22791 GRP94 antibody [9G10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 92 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | heat shock protein 90 beta family member 1 , ECGP , GP96 , GRP94 , HEL-S-125m , HEL35 , TRA1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rat |
| Clone: | 9G10 |
| Isotype: | IgG2a |
| Immunogen: | Chick oviduct GRP94. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC |
Description
Description: This gene encodes a member of a family of adenosine triphosphate(ATP)-metabolizing molecular chaperones with roles in stabilizing and folding other proteins. The encoded protein is localized to melanosomes and the endoplasmic reticulum. Expression of this protein is associated with a variety of pathogenic states, including tumor formation. There is a microRNA gene located within the 5' exon of this gene. There are pseudogenes for this gene on chromosomes 1 and 15. [provided by RefSeq, Aug 2012]
Additional Text
Gene Name
HSP90B1
Gene ID
7184
Uniprot ID
P14625
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
92
Application Notes
WB: 1:100-1:1000. ICC/IF: 1:250. IHC-P: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX22791 Datasheet
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