PDI antibody [RL90]
Catalogue Number: GTX22792-GTX
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IHC-P analysis of human lung adenocarcinoma tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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IHC-P analysis of human colon tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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IHC-P analysis of human tonsil tissue using GTX22792 PDI antibody [RL90]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
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FACS analysis of Hela cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of K562 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of NIH/3T3 cells using GTX22792 PDI antibody [RL90] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde
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WB analysis of 25 ug of HepG2 (Lane 1), Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates using GTX22792 PDI antibody [RL90]. Dilution : 1:1000
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ICC/IF analysis of NIH-3T3 cells using GTX22792 PDI antibody [RL90]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature Dilution : 1:75 for at least 1 hour at room temperature
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ICC/IF analysis of MDCK cells using GTX22792 PDI antibody [RL90]. Permeabilization : 0.1% Triton X-100 Dilution : 10 µg/ml in blocking buffer for at least 1 hour at room temperature
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ICC/IF analysis of A549 cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei
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ICC/IF analysis of HMVEC Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei
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ICC/IF analysis of NS-1 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei
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ICC/IF analysis of p19 Cells using GTX22792 PDI antibody [RL90]. Green : Primary antibody Blue : Nuclei
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ICC/IF analysis of U2OS cells using GTX22792 PDI antibody [RL90]. Red : Primary antibody Green : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in PBS for 10 minutes at room temperature Dilution : 1:75 for at least 1 hour at room temperature
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 57 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | prolyl 4-hydroxylase subunit beta , PDI , PDIR |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | RL90 |
| Isotype: | IgG2a |
| Immunogen: | Purified rat PDI protein. |
| Application: | FC, ICC, IF, IHC-P, IP, WB, IHC-Fr, NT, Inh, IM |
Description
Description: may be identical to protein disulfide isomerase, iodothyronine 5 monodeiodinase (5MD), and membrane associated thyroid hormone binding protein MTHBP. [RGD, Jun 2010]
Additional Text
Gene Name
P4HB
Molecular Weight
57
Gene ID
25506
Purification
Protein A purified
Uniprot ID
P04785
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:200-1:2000. ICC/IF: 1:100. IHC-P: 1:100. IHC-Fr: 1:100. FCM: 0.5-1 µg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22792 Datasheet
SUPPORT
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DELIVERY
we offer free delivery to UK universities and non profit organisations