Importin beta 1 antibody [3E9]
Catalogue Number: GTX22811-GTX
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FACS analysis of Jurkat cells using GTX22811 Importin beta 1 antibody [3E9] compared to an isotype control (blue). Dilution : 1 ug/test for 40 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of NIH-3T3 cells using GTX22811 Importin beta 1 antibody [3E9] compared to an isotype control (blue). Dilution : 1 ug/test for 40 min at room temperature Fixation : 2% paraformaldehyde
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FACS analysis of PC12 cells using GTX22811 Importin beta 1 antibody [3E9] compared to an isotype control (blue). Dilution : 1 ug/test for 40 min at room temperature Fixation : 2% paraformaldehyde
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WB analysis of 25 ug of U251 (lane 1), HepG2 (lane 2) and C6 (lane 3) cell lysates using GTX22811 Importin beta 1 antibody [3E9]. Dilution : 1:50
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ICC/IF analysis of PTK cells using GTX22811 Importin beta 1 antibody [3E9].
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ICC/IF analysis of HMVEC Cells using GTX22811 Importin beta 1 antibody [3E9].
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The data was published in the journal PLoS One in 2017. PMID: 28873410
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The data was published in the journal J Biomed Sci in 2015.PMID: 25981436
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 97 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | karyopherin subunit beta 1 , IMB1 , IPO1 , IPOB , Impnb , NTF97 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Mouse |
| Clone: | 3E9 |
| Isotype: | IgG2a |
| Immunogen: | Purified NTF97 from bovine erythrocytes. |
| Application: | FC, ICC, IF, IP, ChIP, WB, IHC, NT, Inh |
Description
Description: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
Additional Text
Gene Name
KPNB1
Uniprot ID
Q14974
Gene ID
3837
Purification
Protein A purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
97
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:5,000. ICC/IF: 1:1000. FCM: 1 µg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22811 Datasheet
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