Hydrogen Potassium ATPase Beta antibody [2G11]
Catalogue Number: GTX22866-GTX
GTX22866 Image
GTX22866 Image
GTX22866 Image
GTX22866 Image
IHC-P analysis of human gastric carcinoma tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GTX22866 Image
IHC-P analysis of human stomach tissue using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
GTX22866 Image
ICC/IF analysis of A2058 Cells using GTX22866 Hydrogen Potassium ATPase Beta antibody [2G11]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 33 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | ATPase H+/K+ transporting subunit beta , ATP6B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Mouse |
| Clone: | 2G11 |
| Isotype: | IgG1 |
| Immunogen: | Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. |
| Application: | FC, ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr |
Description
Description: The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
Additional Text
Gene Name
ATP4B
Uniprot ID
P51164
Gene ID
496
Purification
Unpurified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
33
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:4,000. ICC/IF: 1:2,000. IHC-P: 1:2000. IHC-Fr: 1:2000. FCM: 1/50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX22866 Datasheet
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